Iron In Antioxidant Antimutagenic Antiaging Antiinflammtory Activities

[ironjustice]

Inhibition of lipoxygenase by sesamol corroborates its potential anti-inflammatory activity.
Yashaswini PS1, Rao AG16
Jun 14. pii: S0141-8130(16)30586-4.

Abstract
Reactive oxygen species, the byproducts of oxygenases reaction, when in excess, promote degenerative diseases like cardiovascular, cancer and arthritis. Sesame lignans- sesamin, sesamolin and the phenolic degradation product of sesamolin, sesamol, are empirically known for thier health promoting properties like antioxidant, antimutagenic, antiaging and antiinflammtory activities. In the current study, the effect of sesamol on the inflammatory oxygenase - lipoxygenase (LOX) was investigated. Enzyme kinetics and spectroscopic techniques were used to understand the inhibition mechanism. Sesamol was a potent inhibitor of soy LOX-1. It inhibited soy LOX-1 in a dose dependent manner with IC50 value of 51.84μM and Ki of 4.9μM. Binding studies using circular dichroism and corroborated by surface plasmon resonance, revealed that sesamol does not bind or change the conformation of LOX. Further, sesamol prevented the conversion of inactive LOX (Fe2+) to active LOX (Fe3+) by arresting the oxidation state of iron and prolonging the lag phase by virtue of its ability to scavenge hydroperoxides. Understanding the mechanism of action of such molecules will help in their application and promotion as nutraceuticals.

Copyright © 2016. Published by Elsevier B.V.

KEYWORDS:
Sesamol; inhibition studies; lipoxygenase
doi: 10.1016/j.ijbiomac.2016.06.048.

PMID: 27316769

Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/a3cc3


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

[Taka]

Post by ironjustice
Inhibition of lipoxygenase by sesamol corroborates its potential anti-inflammatory activity.
Yashaswini PS1, Rao AG16
Jun 14. pii: S0141-8130(16)30586-4.
Abstract
Reactive oxygen species, the byproducts of oxygenases reaction, when in excess, promote degenerative diseases like cardiovascular, cancer and arthritis. Sesame lignans- sesamin, sesamolin and the phenolic degradation product of sesamolin, sesamol, are empirically known for thier health promoting properties like antioxidant, antimutagenic, antiaging and antiinflammtory activities. In the current study, the effect of sesamol on the inflammatory oxygenase - lipoxygenase (LOX) was investigated. Enzyme kinetics and spectroscopic techniques were used to understand the inhibition mechanism. Sesamol was a potent inhibitor of soy LOX-1. It inhibited soy LOX-1 in a dose dependent manner with IC50 value of 51.84μM and Ki of 4.9μM. Binding studies using circular dichroism and corroborated by surface plasmon resonance, revealed that sesamol does not bind or change the conformation of LOX. Further, sesamol prevented the conversion of inactive LOX (Fe2+) to active LOX (Fe3+) by arresting the oxidation state of iron and prolonging the lag phase by virtue of its ability to scavenge hydroperoxides. Understanding the mechanism of action of such molecules will help in their application and promotion as nutraceuticals.
Copyright © 2016. Published by Elsevier B.V.
Sesamol; inhibition studies; lipoxygenase
doi: 10.1016/j.ijbiomac.2016.06.048.
PMID: 27316769
Who loves ya.
Tom
Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh
Man Is A Herbivore!
http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

Nice find Tom, another weapon from the sesame arsenal in our chemical war against the arachidonic acid, in addition to Sesamin which is delta 5-desaturase inhibitor. How smart the Japs are using sesame oil for cooking ;-)